Journal: Molecular endocrinology (Baltimore, Md.)

Article Title: Identification of genes regulated by leukemia-inhibitory factor in the mouse uterus at the time of implantation.

doi: 10.1210/me.2004-0110

Figure Lengend Snippet: Fig. 1. Regulation of Uterine IGFBP3 mRNA Expression by LIF Recombinant LIF was injected into one horn and PBS into the other horn of LIF/ females at 84 h of pseudopregnancy (n 10). IGFBP3 mRNA expression levels were determined in total uterine RNA isolated 12 h later by real-time RT-PCR. Values for IGFBP3 are normalized to 18S rRNA for each horn and expressed in arbitrary units relative to the level of the same gene in a standard RNA produced from mouse uterus. LIF treatment (solid bars) produced increased IGFBP3 expression in nine of 10 animals compared with the paired PBS-treated uterine horn (open bars). The mean increase was 2.3-fold and was statistically significant (P 0.02, paired Mann-Whitney).

Article Snippet: Two ip injections each of 5 g recombinant human LIF (R&D systems 250-LF) in PBS were given at 0900 h and 1600 h on d 4 of pregnancy, and the animals were allowed to proceed to term.

Techniques: Expressing, Recombinant, Injection, Isolation, Quantitative RT-PCR, Produced, MANN-WHITNEY

Journal: Molecular endocrinology (Baltimore, Md.)

Article Title: Identification of genes regulated by leukemia-inhibitory factor in the mouse uterus at the time of implantation.

doi: 10.1210/me.2004-0110

Figure Lengend Snippet: Fig. 6. LIF Regulation of Amphiregulin and IRG1 in the Uterus Recombinant LIF was injected into one horn and PBS into the other horn of LIF/ females at 84 h of pseudopreg- nancy (n 10, same animals as shown in Fig. 1). Amphiregu- lin and IRG1 mRNA expression levels were determined in total uterine RNA isolated 12 h later by real-time RT-PCR. Values for each mRNA species are normalized to 18S rRNA for each horn and expressed in arbitrary units relative to the level of the same gene in a standard RNA produced from mouse uterus. LIF treatment (solid bars) increased both am- phiregulin and IRG1 expression in 10 of 10 animals compared with the corresponding PBS-treated horn (open bars). The mean increase for amphiregulin was 7.3-fold and for IRG1 was 3.7-fold and was statistically significant for either gene (P 0.002, paired Mann-Whitney test).

Article Snippet: Two ip injections each of 5 g recombinant human LIF (R&D systems 250-LF) in PBS were given at 0900 h and 1600 h on d 4 of pregnancy, and the animals were allowed to proceed to term.

Techniques: Recombinant, Injection, Expressing, Isolation, Quantitative RT-PCR, Produced, MANN-WHITNEY

Journal: Molecular Medicine Reports

Article Title: Expression and effects of leukemia inhibitory factor on nucleus pulposus degeneration

doi: 10.3892/mmr.2019.9874

Figure Lengend Snippet: Protein expression of aggrecan and COL2α1 in human nucleus pulposus cells stimulated with various concentrations of LIF at different durations. (A and B) Aggrecan and COL2α1 protein expression in human nucleus pulposus cells treated with various concentrations of recombinant human LIF protein. (C and D) Protein expression levels of aggrecan and COL2α1 in human nucleus pulposus cells treated with 100 ng/ml recombinant human LIF protein for various durations. (E) Toluidine blue staining following treatment of human nucleus pulposus cells with 0 or 100 ng/ml recombinant human LIF protein for 48 h. Data are presented as the means ± standard deviation. *P<0.05. COL2α1, collagen type II α1; LIF, leukemia inhibitory factor.

Article Snippet: Recombinant Human LIF Protein was purchased from Novus Biologicals, LLC (Littleton, CO, USA).

Techniques: Expressing, Recombinant, Staining, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Expression and effects of leukemia inhibitory factor on nucleus pulposus degeneration

doi: 10.3892/mmr.2019.9874

Figure Lengend Snippet: Effects of LIF on the proliferation and apoptosis of nucleus pulposus cells. (A and B) Apoptotic rate of human nucleus pulposus cells treated with various concentrations of recombinant human LIF. (C) Proliferation of human nucleus pulposus cells treated with various concentrations of recombinant human LIF. Data are presented as the means ± standard deviation. *P<0.05. FITC, fluorescein isothiocyanate; LIF, leukemia inhibitory factor; OD, optical density.

Article Snippet: Recombinant Human LIF Protein was purchased from Novus Biologicals, LLC (Littleton, CO, USA).

Techniques: Recombinant, Standard Deviation

Journal: Frontiers in Oncology

Article Title: Next-Generation Sequencing Analysis of Gastric Cancer Identifies the Leukemia Inhibitory Factor Receptor as a Driving Factor in Gastric Cancer Progression and as a Predictor of Poor Prognosis

doi: 10.3389/fonc.2022.939969

Figure Lengend Snippet: LIFR activation promotes cell proliferation and EMT in MKN45 cells. Relative mRNA expression (A) LIFR and (B) LIF in CG cell lines. (C) IHC staining of LIFR in MNK45 cell lines on the left untreated and on the right triggered with LIF (10 ng/ml; magnification, ×100). MKN45 cells were serum-starved and primed with LIF (0.5, 5, and 10 ng/ml). Data shown are as follows: (D) dose–response curve of LIF (0.5, 5, and 10 ng/ml) determined using MTS assay on MKN45 cells. Each value is expressed relative to those of non-treated (NT), which are arbitrarily settled to 1. Results are the mean ± SEM of 10 samples per group. Relative mRNA expression of (E) the proliferation marker C-Myc and EMT markers (F) E-cadherin, (G) vimentin, and (H) Snal-1. Each value is normalized to Gapdh and is expressed relative to those of positive controls, which are arbitrarily settled to 1. Results are the mean ± SEM of five samples per group (* represents statistical significance versus NT, and # versus LIF, p < 0.05).

Article Snippet: Immunocytochemistry (ICC) was performed on MKN45, untreated or treated with LIF (10 ng/ml; 14890-H02H, SinoBiological, Düsseldorfer, 65760 Eschborn, Germany).

Techniques: Activation Assay, Expressing, Immunohistochemistry, MTS Assay, Marker

Journal: Frontiers in Oncology

Article Title: Next-Generation Sequencing Analysis of Gastric Cancer Identifies the Leukemia Inhibitory Factor Receptor as a Driving Factor in Gastric Cancer Progression and as a Predictor of Poor Prognosis

doi: 10.3389/fonc.2022.939969

Figure Lengend Snippet: LIFR antagonist EC359 hinders cell cycle progression, increases apoptosis rate in MKN45 cells and inhibits EMT process. (A) Dose–response curve of EC359 (25, 50, 100, and 1,000 nM) determined using MTS assay on MKN45 cells (n = 10). MKN45 cells were serum-starved and triggered with LIF (10 ng/ml), EC359 (100 nM), and LIF + EC359 for 48 h. Cell cycle phase analysis was performed by Ki-67/DAPI staining through IC-FACS. Data shown are follows: percentage of (B) from left to right cell in G0-G1 cell cycle phases, S-G2-M cell cycle phases, and ratio between % G0-G1 and % S-G2-M. (C) Percentage of apoptotic cells. (D) Representative IC-FACS showed cell cycle fraction and apoptosis rate in NT, LIF (10 ng/ml), EC359 (25 nM), and LIF + EC359. Results are the mean ± SEM of three samples for group (* represents statistical significance versus NT, and # versus LIF, p < 0.05). Relative mRNA expression of (E) the proliferation marker C-Myc and EMT markers (F) E-Cadherin, (G) Snal-1, and (H) vimentin. Each value is normalized to Gapdh and is expressed relative to those of positive controls, which are arbitrarily settled to 1. Results are the mean ± SEM of five samples per group (* represents statistical significance versus NT, and # versus LIF, p < 0.05).

Article Snippet: Immunocytochemistry (ICC) was performed on MKN45, untreated or treated with LIF (10 ng/ml; 14890-H02H, SinoBiological, Düsseldorfer, 65760 Eschborn, Germany).

Techniques: MTS Assay, Staining, Expressing, Marker

Journal: Frontiers in Oncology

Article Title: Next-Generation Sequencing Analysis of Gastric Cancer Identifies the Leukemia Inhibitory Factor Receptor as a Driving Factor in Gastric Cancer Progression and as a Predictor of Poor Prognosis

doi: 10.3389/fonc.2022.939969

Figure Lengend Snippet: Analysis of JAK-STAT signaling pathway. Representative Western blot analysis of (A) LIFR, JAK1 and phospho-JAK1, STAT3 and phospho-STAT3, and proteins in MKN45 exposed to LIF (10 nM) alone or in combination with EC359 (25 nM and 100 nM) for 20 min. GAPDH was used as loading control. (B) Densitometric analysis demonstrating LIFR expression, phospho-JAK1/JAK1, and phospho-STAT3/STAT3 ratio. The blot shown is representative of another one showing the same pattern. (* represents statistical significance versus NT, and # versus LIF, p < 0.05).

Article Snippet: Immunocytochemistry (ICC) was performed on MKN45, untreated or treated with LIF (10 ng/ml; 14890-H02H, SinoBiological, Düsseldorfer, 65760 Eschborn, Germany).

Techniques: Western Blot, Expressing

Journal: Frontiers in Oncology

Article Title: Next-Generation Sequencing Analysis of Gastric Cancer Identifies the Leukemia Inhibitory Factor Receptor as a Driving Factor in Gastric Cancer Progression and as a Predictor of Poor Prognosis

doi: 10.3389/fonc.2022.939969

Figure Lengend Snippet: LIFR antagonism inhibits MKN45. (A, B) A scratch wound healing assay is shown. MKN45 cell monolayers were scraped in a straight line using a p200 pipette tip to create a “scratch”, and then, they are left untreated or primed with LIF (10 ng/ml), EC359 (100 nM), and LIF + EC359. The wound generated was imaged at 0, 24, and 48 h of incubation with the indicated compounds. (A) The images show cell migration at the three times point indicated. (B) Images obtained points were analysed measuring scraped area and its closure vs. the first time point at 0 h. Results are the mean ± SEM of three samples per group. (C) Cell adhesion to peritoneum. Experiment was conducted in quintuplicate (* represents statistical significance versus NT, and # versus LIF, p < 0.05).

Article Snippet: Immunocytochemistry (ICC) was performed on MKN45, untreated or treated with LIF (10 ng/ml; 14890-H02H, SinoBiological, Düsseldorfer, 65760 Eschborn, Germany).

Techniques: Wound Healing Assay, Transferring, Generated, Incubation, Migration

Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

Article Title: Stachydrine hydrochloride inhibits hepatocellular carcinoma progression via LIF/AMPK axis.

doi: 10.1016/j.phymed.2022.154066

Figure Lengend Snippet: Fig. 4. SH triggers autophagic and se nescent capacities and induces cell- cycle arrest via LIF/AMPK axis. (A, B, C) Docking model of SH with LIF. (D) Binding ability of SH and LIF was detected by CETSA experiment. And the protein abundance of LIF was shown by Western blot, which relative band abundance was indicated as scattered graph. (E) The protein expression of LIF was detected in HCC cells intervened with SH (0, 40, 80, 120, 160 μM) for 48 h. (F) The protein expression of AMPK and p-AMPK were detected in HCC cells administrated with indicated doses of SH (0, 40, 80, 120, 160 μM) for 48 h. (G) The protein expression of AMPK and p-AMPK were detected by Western blot under conditions in which LIF was overexpressed. (H) The protein expres sion of LC-3B, p27 and p21 were tested by Western blot under conditions in which LIF was overexpressed.

Article Snippet: Antibodies against LC-3B (3868), p62 (8025), Cyclin D1 (2978T), LIF (62226) were got from Cell Signaling Technology (MA, USA).

Techniques: Binding Assay, Quantitative Proteomics, Western Blot, Expressing